Cell-Based & Mechanistic Molecular Assays

Our comprehensive cell-based and mechanistic molecular assay platforms enable detailed functional analyses of gene regulation, cellular signaling, and response dynamics pivotal for drug discovery and translational research. Harnessing a diverse toolkit that spans reporter gene systems, pathway-specific monitoring, advanced gene delivery, and single-cell genomics, we deliver insights that transform basic biological understanding into therapeutic innovation.

We employ versatile reporter systems—including luciferase, secreted embryonic alkaline phosphatase (SEAP), NanoLuc, and green fluorescent protein (GFP)—to quantify cellular responses at transcriptional and post-transcriptional levels. These highly sensitive assays facilitate pathway interrogations, drug screening, and gene promoter activity assessments in a high-throughput compatible format.

Our dedicated platforms measure activation and modulation of critical pathways such as NF-κB, CREB, MAPK, Wnt, and G-protein-coupled receptors (GPCRs) via phosphorylation status, reporter outputs, and downstream expression analyses. This comprehensive pathway profiling deciphers molecular mechanisms underlying disease and therapeutic action.

We offer optimized gene delivery strategies tailored to maximize transfection efficiency with minimal cytotoxicity across cell types. Techniques including lipofection, electroporation, and viral transduction are validated to ensure consistent gene expression needed for functional assays and mechanistic studies.

Our cutting-edge single-cell genomic assays (single-cell qPCR, flow-based RNA analysis) enable profiling of cellular heterogeneity and gene expression at unprecedented resolution. Coupled with CRISPR activation/interference (CRISPRa/i) technologies, we perform targeted gene function mapping and rescue assays, unraveling regulatory networks and phenotype-genotype relationships.