Molecular Biology In Vitro Assays

Accelerate your genomic and transcriptomic research projects with our comprehensive nucleic acid analysis portfolio. From pristine DNA/RNA purification to cutting-edge gene editing validation, our customizable workflows and state-of-the-art technologies ensure reproducibility, sensitivity, and regulatory compliance—empowering discovery, translational research, and clinical validation.

We extract and purify high-integrity DNA, RNA, and plasmids from a broad range of biological sources and sample types. Our standardized protocols optimize yield and purity, verified through quantitative and qualitative assessments—including spectrophotometry, fluorometry, and gel electrophoresis—to ensure reliable downstream assay performance.

Comprehensive platforms encompassing RT-PCR, qPCR, ddPCR, and isothermal amplification techniques such as LAMP, RPA, and SDA provide unparalleled precision in quantifying gene expression and detecting low-abundance nucleic acid targets. These assays support everything from routine expression profiling to point-of-care diagnostics.

Our FISH and Flow-FISH techniques allow visualization and quantification of specific DNA/RNA sequences in cells with spatial resolution crucial for cytogenetic and molecular investigations. Complementary Southern and Northern blot analyses deliver size and abundance insights critical for detailed molecular characterizations.

We provide robust validation for gene editing and silencing tools—CRISPR/Cas, TALENs, siRNA, and shRNA—using PCR, qPCR, and Western blot endpoints. Our assays quantify editing efficiency and off-target effects, helping clients confidently advance functional studies and therapeutic development.

Advanced genetic and epigenetic interrogation includes TaqMan SNP genotyping, High-Resolution Melt (HRM) analysis, Methylation-Specific PCR (MSP-PCR), and CpG enzyme restriction assays. These methods reveal critical regulatory modifications linked to disease and therapeutic response.