Regulated Cell Death Assays

Understanding regulated cell death is fundamental for innovative drug development, disease modeling, and toxicological safety assessment. Our comprehensive suite of assays accurately detects and quantifies diverse cell death pathways—apoptosis, autophagy, ferroptosis, pyroptosis, and necroptosis—empowering clients with mechanistic insights critical to advancing therapeutics and safety evaluation.

Rapidly identify apoptotic cells with our Annexin V/Propidium Iodide (PI) dual-staining protocols that differentiate early apoptotic, late apoptotic/necrotic, and viable cells through flow cytometry or imaging.

Complement with TUNEL assay, which detects DNA fragmentation, providing spatial resolution of apoptosis in fixed cells or tissue samples. These gold-standard methods deliver clarity on cell death stage and extent.

Gain mechanistic insights into intrinsic and extrinsic apoptosis by measuring caspase enzymatic activity with high sensitivity assays, critical for understanding drug-induced cell death pathways.

Evaluate mitochondrial integrity using dyes such as JC-1 and TMRM that report on membrane potential changes, an early apoptotic event, offering a window into mitochondrial health and dysfunction.

Expand your understanding beyond classical apoptosis with assays targeting ferroptosis, characterized by iron-dependent lipid peroxidation; pyroptosis, detected by Gasdermin D (GSDMD) cleavage indicative of inflammatory cell death; and necroptosis, with markers such as RIPK1/3 and MLKL activation.

These assays unlock new dimensions in cell death research relevant to cancer, immunology, and neurodegeneration.

Quantify autophagic flux by assessing LC3-II conversion and p62 degradation via immunoblot or fluorescence imaging.

Track mitophagy dynamically using mito-Keima, a pH-sensitive fluorescent reporter that distinguishes mitochondria degradation within lysosomes, providing insights into cellular quality control and stress responses.